Molecular formula: C20H26N2O5S

 Molecular weight: 406.50

CAS Registry No: 74258-86-9

 Merck Index: 13, 200 H3C S N O CH3 O O N H COOH

SAMPLE Matrix: solutions HPLC VARIABLES

 

*** Column: 250 × 4.6  5μm Cosmosil 5C18-MS

Column temperature: 50

Mobile phase: Gradient. MeCN:10 mM pH 2.5 potassium phosphate buffer 0:100 for 2 min, to 75:25 over 7.5 min, maintain at 75:25 for 5.5 min.

Isocratic. MeCN:buffer 40:60

 Flow rate: 1.5

Detector: UV (wavelength not specified)

 

CHROMATOGRAM Retention time: 10.9 (gradient) or 4.1 (isocratic) OTHER SUBSTANCES Simultaneous: acetaminophen (7.9), ampicillin (7.9), aspirin (10.0), caffeine (8.5), carbenicillin (9.5), cefotiam (7.2), chlorpromazine (10.8), cromolyn (8.9), enalapril (9.9), loperamide (11.6), ofloxacin (8.3), procainamide (7.4), procaine (7.9), propranolol (9.6), sultamicillin tosylate (8.3), tegafur (8.4), temocapril (12.3), theophylline (8.0), tulobuterol (8.9) (gradient retention times; isocratic conditions may differ)

 

 REFERENCE Sugiyama, T.; Matsuyama, R.; Usui, S.; Katagiri, Y.; Hirano, K. Selection of mobile phases in highperformance liquid chromatographic determination for medicines, Biol.Pharm.Bull., 2000, 23, 274–278.

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SAMPLE Matrix: enzyme reactions

Sample preparation: Mix 40 μL enzyme reaction mixture with 200μLMeCN,add 200 μLofa20μg/mL solution of n-propyl paraben, centrifuge, inject a 30 μL aliquot of the supernatant.

HPLC VARIABLES Column: 250 × 4.6  Cosmosil5-C18MS

 Column temperature: 50

 Mobile phase: MeCN:10 mM pH 2.5 potassium phosphate buffer 40:60

Flow rate: 1.5

Injection volume: 30

 Detector: UV 220

CHROMATOGRAM Internal standard: n-propyl paraben